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reverse phase hplc中文是什么意思

  • 反相高压液相层析

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  • The method for the determination of amino acid neurotransmitters in brain by reverse phase hplc system with the precolumn phthali aldehyde derivatization
    柱前衍生反相高效液相色谱法测定脑组织中氨基酸类神经递质
  • It is introduced that the quantitative analysis of trichlorfon by reverse phase hplc , using acetonitrile and water ( 50 : 50 ) as mobile phase , using cn column and ultraviolet detector
    摘要本文采用即以乙腈+水( 50 + 50 )为流动相,用cn柱和紫外检测器分离测定敌百虫原药及40 %敌?毒乳油中敌百虫反相高效液相色谱法。
  • The quantitative analysis of 25 % chlorpyrifos and triazophos ec by reverse phase hplc was introduced , using acetonitrile and water ( 74 : 26 ) as mobile phase , using c18 column and ultraviolt detector
    摘要本文叙述了采用反相高效液相色谱法即以乙腈+水为流动相, c18柱和紫外检测器同柱分离侧定25 %毒?唑乳油中的毒死蜱和三唑磷。
  • Imidacloprid and triazophos in mixture were determined simultareously by reverse phase hplc , methanol and acetonitrile and water and glacial acetic acid as mobile phase , c18 column and uv200 detecter
    摘要采用c18不锈钢柱和uv200型检测器,以甲醇?乙腈?水的混合液为流动相,用反相高效液相色谱法对吡虫啉?三唑磷复配制剂进行定量分析测定。
  • The crude venom contains a mixture of compounds with different types of biological activities . two novel peptide neueotoxins were purified from the venom of chilobrachys jingzhao by using combination of ion - exchange and reverse phase hplc
    海南敬钊缨毛蛛( chilobrachysjingzhao )毒素是最近在海南发现的蜘蛛新种,该蜘蛛粗毒中含有多种活性多肽和蛋白质成分。
  • Secondly , determination of the antimicrobial activities of synthetic hpab - . i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc . the molecular weight of synthesis peptide was 4334 . 96da characterized by mass spectrometry , highly agreed with the theoretical mw of hpab - 3
    2 . hpab一p抗菌活性初步鉴定:采用固相法合成了hpab一p成熟肤,经即一hplc纯化后用质谱测定合成肤的分子量为4334 . % da ,与其理论分子量相符。
  • Both hainantoxin - iv ( hntx - iv ) and . hainantoxin - v ( hntx - v ) w ere peptide toxins isolated from the venom of the spider seleconosmia hainana by means of ion - exchange chromatograph and reverse phase hplc . hntx - iv , 3989 da , contained 35 amino acid residues with three disulfide bonds , while the molecular weight of hntx - v was 3972 da and had three disulfide bonds , too
    海南捕鸟蛛毒素- ( hntx - )和海南捕鸟蛛毒素- ( hntx - )是从海南捕鸟蛛粗毒中经阳离子交换和反相hplc柱层析分离出来的两种神经毒素, maldi - tof质谱鉴定前者相对分子质量为3 , 989da ,含有三对二硫键,由35个氨基酸残基组成;后者相对分子质量为3 , 972da ,与前者只相差16个质子,也含有三对二硫键。
  • The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
    将带有hwtx -基因的ppic9k经blgii线性化后,转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌,进一步用遗传毒素g418筛选多拷贝的转化菌株,命名为gh1 ;将gh1甲醇酵母菌用0 . 5的甲醇诱导表达,发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀, yw - 3 ( mwc03000 )的超滤膜超滤,再经cm阳离子交换, c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分,其中4289 . 05的组分经质谱鉴定,氨基酸组成分析和序列测定为正确的表达产物,生物学活性表明其活性为天然毒素活性70 % ,表达量为80mg / l 。
  • A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc . hwtx - v has 35 amino acid residues , with the molecular mass 4111 . 4da . the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh , which consists of 6 cys , formed three pairs of disulfide bridges
    本文报道从虎纹捕鸟蛛( selenocoimahuwena )粗毒中,结合阳离子交换和反相高效液相色谱分离纯化出一种昆虫毒素,命名为虎纹捕鸟蛛毒素- ( huwentoxin - , hwtx - ) ;其天然突变体( themutantofhuwentoxin , mhwtx - )也同时纯化出来。
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Last modified time:Sun, 10 Aug 2025 00:29:56 GMT

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